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Image Search Results
Journal: Molecular Cell
Article Title: Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability
doi: 10.1016/j.molcel.2018.11.036
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Modification, Protease Inhibitor, Mutagenesis, Transfection, Labeling, Purification, Multiplex Assay, Sequencing, Derivative Assay, Clone Assay, esiRNA, Negative Control, Software
Journal: bioRxiv
Article Title: Chromatin-associated microprocessor assembly is regulated by PRP40, the U1 snRNP auxiliary protein
doi: 10.1101/2022.01.31.478465
Figure Lengend Snippet: a , Close proximity of AtPRP40b and SE in the cell nucleus (first image, red signals) analyzed by PLA in view of SE nuclear localization (second image, green signals). DNA was stained with Hoechst (blue). Scale bar = 5 µm. b , Close proximity of SE and RNAPII phosphorylated at CTD Ser5 or Ser2 in the WT, prp40ab and se-2 plants (first columns, red signals) in view of P-Ser5-RNAPII or P-Ser2-RNAPII nuclear localization (second columns, green signals) detected by PLA. DNA was stained with Hoechst (blue). Scale bar = 5 µm. c , Close proximity of AtPRP40b and RNAPII phosphorylated at CTD Ser5 or Ser2 in the WT, se-2 and prp40ab plants (first columns, red signals) in view of P-Ser5-RNAPII or P-Ser2-RNAPII nuclear localization (second columns, green signals) detected by PLA. DNA was stained with Hoechst (blue). Scale bar = 5 µm. d , In vitro pull-down assays using recombinant AtPRP40b and SE proteins (both full length and the shortened C-terminus variant b.681-720 were used) and CTD peptides in unphosphorylated (UnP) or phosphorylated Ser5 (P-Ser5-CTD) or Ser2 (P-Ser2-CTD) forms. Input represents 1/10 of the protein sample.
Article Snippet: The following antibodies were used:
Techniques: Staining, In Vitro, Recombinant, Variant Assay